肿瘤微环境，DNA损伤修复及肿瘤治疗，生物信息及人工智能分析

主持科研项目：

1. 国家自然科学基金面上项目，RNF8缺失诱导成纤维细胞衰老促进黑色素瘤进展的机制研究，2026.1-2029.12，主持，在研

2. 国家自然科学基金青年项目，RNF8经调控galectin-3泛素化修饰影响黑色素瘤免疫微环境的机制研究，2023.1-2025.12，主持，结项

3. 兰州市青年科技人才创新项目，肿瘤微环境重塑对肿瘤转移影响的研究， 2023.11-2025.10，主持，结项

4. 兰州大学中央高校定向探索项目，RNF8经GAPDH介导瘤内成纤维细胞代谢重编程影响黑色素瘤转移机制的研究，2022.7-2024.6，主持，结项

5. 横向项目，RNF8在ACSL4介导的肝脏脂代谢中的作用及调控机制研究， 2022.4-2024.4，主持，结项

6. 科技部重点研发项目，裸鼹鼠、长爪沙鼠等五种特色实验动物的辐射损伤、代谢性紊乱、抗血吸虫病、帕金森病、脑缺血等疾病模型的创建及关键技术研究， 2022.1-2026.11，参与，在研

7. 国家自然科学基金面上项目，泛素连接酶RNF8通过调控UQCRC2影响精子活力的机制研究， 2021.1-2024.12，参与，结项

8. 国家自然科学基金面上项目，RAD6B通过组蛋白翻译后修饰调节Snail1转录及结肠癌转移机制的研究， 2021.1-2024.12，参与，结项

9. 甘肃省普通中医药科研课题，当归补血汤抗放射性心肌损伤的物质基础及作用机制研究， 2023.1-2026.12，参与，在研

10. 兰州大学科技创新平台建设项目，基于微环境的肿瘤免疫治疗研究， 2022.11-2024.12，参与，结项

11. 甘肃省自然科学基金，结合拉曼光谱技术研究RNF8基因对脂代谢的调控机制， 2021.11-2023.11，主持，结项

12. 兰州大学中央高校定向探索项目，共焦拉曼光谱仪在检测重金属镉所致肾损伤中的应用， 2018.1-2019.12，主持，结项

近五年代表性论文：

1. Wang J^#, Dong WH^#, Zhang JR, Liu LY, Ji PF, Yuan XY, Shuang S, Fu DS, Guo Z, Tian YX^*, Shen R^*, Wang DG^*. Hypoxia-Responsive Nanosystem for Synergistic Cancer Therapy: Enhancing Ferroptosis via a Novel GPX4 Inhibitor and Facilitating Precise Photodynamic Therapy. Advanced Functional Materials. 2026 Mar, e29287.

2. Hu XH^#, Tian YX^#, Chen QP, Guo YN, Ji PF, Tian YX, Qian SX, Chai GL, Hu FD^*, Shen R^*, Wang DG^*. Flavonoid Compounds from Shenrong Guben Huanshao Pill alleviates senescence-associated mitochondrial dysfunction through NDUFV2 and LDHB mediated metabolic reprogramming. Journal of Pharmaceutical Analysis. 2026 Jan, 101559.

3. Xia P^#, Si Shuang S^#, Fu DS^#, Liu LY, Yang DL, Guo, Tian YX, Ji PF, Yuan XY, Tian YX^*, Shen R^*, Wang DG^*. Large-scale single-cell analysis and in silico perturbation reveal dynamic evolution of HCC: from initiation to therapeutic targeting. NPJ Precis Oncol. 2026 Jan 30;10(1):100.

4. Wang YJ^#, Wu PC^#, Zhang YQ, Lou SK, Zhang HB, Ge K, Zhou L, Liu WS^*, Yang L^*, Shen R^*. Optimization of long afterglow nanoparticles with enhanced photothermal effects: NIR imaging combined with cuproptosis strategy for choroidal melanoma therapy. Talanta. 2025;300(2026)129211.

5. Xia P^#, Zhou J^#, Shen R^*, Wang D^*. Deciphering the cellular and molecular landscape of cervical cancer progression through single-cell and spatial transcriptomics. NPJ Precis Oncol. 2025;9(1):158.

6. Song H^#, Guo Z^#, Xie K, Liu X, Yang X, Shen R^*, Wang D^*. Crotonylation of MCM6 enhances chemotherapeutics sensitivity of breast cancer via inducing DNA replication stress. Cell Prolif. 2025;58(2):e13759.

7. Chen Q, Jin Y, Liao L, Shen R, Ge X, Jiang Q, Huang Y, Sun Q, Liu D, Liu L, Liu T, Dai Q, Tang X, Han Z, Gao X^*, Lin X^*, Mao W^*, Zhu J^*. Patients-Derived Organoids Sequencing-based FOXP4 Facilitates Radioresistance by Transcriptionally Modifying GPX4 to Regulate ferroptosis in Colorectal Cancer. Adv Sci (Weinh). 2025:e07080.

8. Guo Y, Xia P, Tian Y, Fu D, Hu X, Xie K, Dong W, Zhang W, Liu D, Shen R^*, Wang D^*. The RNF8/OPTN/KDM6A axis controls macrophage polarization to maintain testicular microenvironment homeostasis. Cell Death Discov. 2025;11(1):339.

9. Liu L, Lou S, Fu D, Ji P, Xia P, Shuang S, Dong W, Yuan X, Wang J, Xie K, Wang D^*, Shen R^*. Neuro-immune interactions: Exploring the anti-inflammatory role of the vagus nerve. Int Immunopharmacol. 2025;159:114941.

10. Shen R^#, Xia P^#, Guo Y, Ji P, Yuan X, Wang L, Shuang S, Zhou L, Tong R, Zhang L, Liu D^*, Wang D^*. Effects of polystyrene microparticles exposures on spermatogenic cell differentiation and reproductive endpoints in male mice. Environ Pollut. 2025;373:126200.

11. Guo Z^#, Song HY^#, Tian YX^#, Xu J, Zhang GK, Guo YA, Shen R^*, Wang DG^*. siRNF8 Delivered by DNA Framework Nucleic Acid Effectively Sensitizes Chemotherapy in Colon Cancer. INT J NANOMED. 2024;19:171-188.

12. Guo Y^#, Tian Y^#, Xia P, Zhou X, Hu X, Guo Z, Ji P, Yuan X, Fu D, Yin K, Shen R^*, Wang DG^*. Exploring the Function of OPTN From Multiple Dimensions. Cell Biochem Funct. 2024;42(8):e70029.

13. Hu X^#, Yuan X^#, Zhang G, Song H, Ji P, Guo Y, Liu Z, Tian Y, Shen R^*, Wang DG^*. The intestinal epithelial-macrophage-crypt stem cell axis plays a crucial role in regulating and maintaining intestinal homeostasis. Life Sci. 2024:122452.

14. Liu DS, Wang L, Ha WH, Li K, Shen R^*, Wang DG^*. HIF-1alpha: A potential therapeutic opportunity in renal fibrosis. Chem Biol Interact. 2024;387:110808.

15. Guo YA^#, Shen R^#, Wang F^#, Wang YT, Xia P, Wu RL, Liu XW, Ye WC^*, Tian YX^*, Wang DG^*. Carbon ion irradiation induces DNA damage in melanoma and optimizes the tumor microenvironment based on the cGAS-STING pathway. J Cancer Res Clin. 2023;149(9):6315-6328.

16. Zhang GK, Ji PF, Xia P, Song HY, Guo Z, Hu XH, Guo YN, Yuan XY, Song YF, Shen R^*, Wang DG^*. Identification and targeting of cancer-associated fibroblast signature genes for prognosis and therapy in Cutaneous melanoma. Comput Biol Med. 2023 Dec:167:107597.

17. Ji PF, Zhang GK, Guo YA, Song HY, Yuan XY, Hu XH, Guo Z, Xia P, Shen R^*, Wang DG^*. Protein crotonylation: An emerging regulator in DNA damage response. Life Sci. 2023;331.

18. Song HY, Shen R, Mahasin H, Guo YN, Wang DG^*. DNA replication: Mechanisms and therapeutic interventions for diseases. Medcomm. 2023;4(1).

19. Song HY^#, Shen R^#, Liu XW, Yang XG, Xie K, Guo Z, Wang DG^*. Histone post-translational modification and the DNA damage response. Genes Dis. 2023;10(4):1429-1444.

20. Xia P^#, Ouyang SW^#, Shen R, Guo Z, Zhang GK, Liu XW, Yang XG, Xie K, Wang DG^*. Macrophage-Related Testicular Inflammation in Individuals with Idiopathic Non-Obstructive Azoospermia: A Single-Cell Analysis. Int J Mol Sci. 2023;24(10).

21. Shen R, Yang KR, Cheng X, Guo CL, Xing XQ, Sun HN, Liu DS, Liu XW, Wang DG. Accumulation of polystyrene microplastics induces liver fibrosis by activating cGAS/STING pathway. Environ Pollut. 2022; 1:300:118986.

22. Shen R^#, Liu DS^#, Wang XN, Guo Z, Sun HA, Song YF, Wang DG^*. DNA Damage and Activation of cGAS/STING Pathway Induce Tumor Microenvironment Remodeling. Front Cell Dev Biol. 2022;9.

23. Wu DM^#, Li J^#, Shen R^#, Li J, Yu Y, Li L, Deng SH, Liu T, Zhang T, Xu Y, Wang DG^*. Autophagy Induced by Micheliolide Alleviates Acute Irradiation-Induced Intestinal Injury Inhibition of the NLRP3 Inflammasome. Front Pharmacol. 2022;12.

24. Ye Q^#, Wang JQ^#, Liu XW, Liu ZH, BaZong LS, Ma JH, Shen R^*, Ye WC^*, Zhang WF^*, Wang DG^*. The Role of RAD6B and PEDF in Retinal Degeneration. Neuroscience. 2022;480:19-31.

25. Yu LH^#, Shen R^#, Guo YN, Guo YX, Li C, Song YF, Wang DG^*. Loss of the ubiquitin-conjugating enzyme Rad6B disturbs mitochondrial function and cellular homeostasis in mouse skin. Biocell. 2021;45(3):761-772.

26. Yang Y^#, Feng Y^#, Li H, Shen R, Wang YZ, Song XR, Cao C, Zhang GL, Liu WS^*. Hydro-soluble NIR fluorescent probe with multiple sites and multiple excitations for distinguishing visualization of endogenous Cys/Hcy, and GSH. Sensor Actuat B-Chem. 2021;333.

教学研究论文：

1. 王江昆，马智超，王金玉，诸春敏，沈蓉，张朗，王德贵^*. 局部解剖学教学方法研究热点可视化分析[J]. 解剖学研究. 2024,46(3).

2. 诸春敏,沈蓉,王德贵.浅谈提升“局部解剖学”教学质量的主要环节. 教育教学论坛. 2023(10).

3. 王江昆,沈蓉,张朗,洪建平,杨旭光,王德贵.基于数字教育资源的局部解剖学智慧教学探索与实践，中国医学教育技术. 2023,37(05).

4. 沈蓉,王江昆,刘向文,杨旭光,宋焱峰,王德贵.智慧教育在基础医学教育中的运用[J].甘肃教育,2023,724(08):92-96.

5. 沈蓉, 王江昆, 王德贵,杨旭光,诸春敏.线上线下相结合教学模式在局部解剖学中的初探[J].医药卫生,2022(3):0183-0185

专利：

一种检测锌离子的荧光分子探针及其制备方法,2015-8-19,中国, ZL20141021567.8。

一种蕨麻总黄酮提取设备，2020-6-19，中国，ZL201910338979.6。